Expression of microRNA-16-5b, microRNA-27a, -34a-5p, -125b, -181 and -193b in cutaneous melanoma tissue
https://doi.org/10.17650/2219-4614-2024-16-4-100-105
Abstract
Aim. To study the expression of microRNA-16-5b, -27a, -34a-5p, -125b and -193b in melanoma tissue depending on the clinical and morphological characteristics of the tumor.
Materials and methods. The study included 21 patients with a verified diagnosis of cutaneous melanoma. The expression of miR-16-5b, -27a, -34a-5p, -125b and -193b were studied in tumor tissue by real-time polymerase chain reaction. The patients are divided into 5 groups. The group 1 included patients with a tumor <0.75 mm thick (according to the Breslow classification), group 2 – 0.75–1.5 mm, group 3 – 1.51–3 mm, group 4 – 3–4 mm, group 5 – >4 mm. The control group consisted of 10 patients with skin nevi.
Results. The expression of microRNA-27a-3p was reduced in group 5 compared to group 2 by 1.7 times. At the same time, the expression of microRNA-16-5b was, on the contrary, increased by 21.7 times in group 5 compared to groups 3 and 4. Multidirectional changes in microRNA expression were revealed when comparing parameters in groups differing in the depth of invasion. There was a 4.0-fold decrease in the expression of microRNA-27a in the group with Clark invasion III compared to the group with Clark invasion II, which was accompanied by an increase in the expression of microRNA-16-5b and microRNA-125 by 8.7 and 19.8 times, respectively. Moreover, the presence of signs of ulceration was associated with a low level of expression of microRNA-27a-3p against the background of an increase in the expression of the transcription factor microRNA-16-5b and microRNA-34a-5p.
Conclusion. Thus, the features of microRNA expression in melanoma tissue have been identified. Changes in the expression of microRNA-27a-3p, -16-5b, -34a-5p and -125b are associated with the depth of its invasion and the presence of signs of invasive growth.
About the Authors
V. A. Bogdanova
Siberian State Medical University, Ministry of Health of the Russia
Russian Federation
Russian Federation
Veronika Alexandrovna Bogdanova
2 Moskovsky Tract, Tomsk 634050
L. V. Spirina
Siberian State Medical University, Ministry of Health of the Russia; Cancer Research Institute, Tomsk National Research Medical Center of the Russian Academy of Sciences
Russian Federation
Russian Federation
2 Moskovsky Tract, Tomsk 634050
5 Kooperativny Pereulok, Tomsk 634009
S. Yu. Chizhevskaya
Siberian State Medical University, Ministry of Health of the Russia; Cancer Research Institute, Tomsk National Research Medical Center of the Russian Academy of Sciences
Russian Federation
Russian Federation
2 Moskovsky Tract, Tomsk 634050
5 Kooperativny Pereulok, Tomsk 634009
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Review
For citations:
Bogdanova V.A., Spirina L.V., Chizhevskaya S.Yu. Expression of microRNA-16-5b, microRNA-27a, -34a-5p, -125b, -181 and -193b in cutaneous melanoma tissue. Bone and soft tissue sarcomas, tumors of the skin. 2024;16(4):100-105. (In Russ.) https://doi.org/10.17650/2219-4614-2024-16-4-100-105
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