Экспрессия CRABP1 и CRABP2 в саркомах мягких тканей

Цель работы. исследование экспрессии мРНК CRABP1 и CRABP2 и белка CRABP1 в саркомах мягких тканей человека. Материалы и методы. ПЦР в реальном времени, ИГХ анализ. Результаты. В работе впервые проведен анализ экспрессии мРНк генов CRABP1 и CRABP2 методом ПЦР в реальном времени в образцах злокачественных новообразований мягких тканей, включая синовиальные саркомы, злокачественные фиброзные гистиоцитомы, злокачественные шванномы и липосаркомы, а также иммуногистохимический анализ экспрессии белка CRABP1 в образцах монофазных синовиальных сарком. Повышение уровня мРНк как CRABP1, так и CRABP2 было выявлено исключительно в образцах синовиальных сарком и злокачественных фиброзных гистиоцитом (46% для CRABP1 и 54% для CRABP2). Б отличие от синовиальных сарком и злокачественных фиброзных гистиоцитом в липосаркомах и злокачественных шванномах не было отмечено повышения экспрессии ни CRABP1, ни CRABP2; более того, для 40% данных опухолей было характерно снижение количества мРНК CRABP1. По данным ИГХ, высокий уровень белка CRABP1 обнаруживается в 100% образцов монофазных синовиальных сарком. Заключение. Полученные результаты свидетельствуют о дифференциальном характере экспрессии белков CRABP в злокачественных опухолях мягких тканей в зависимости от гистологического типа. Полученные данные открывают перспективы для дальнейших исследований белка CRABP1 в качестве потенциального прогностического маркера и мишени для таргетной терапии синовиальных сарком.

синовиальные саркомы, злокачественные фиброзные гистиоцитомы, липосаркомы, злокачественные шванномы, CRABP1, CRABP2, игх анализ, пЦР в реальном времени, synovial sarcomas, malignant fibrous histiocytomas, malignant schwannomas, liposarcomas, CRABP1, CRABP2, IHC analysis, real-time PCR

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